CELL MORPHOLOGY OF THE DIFFUSE ENDOCRINE SYSTEM OF THE SMALL INTESTINE OF WHITE RATS

Abstract

The aim of this study is to study the morphological and morphometric features of the endocrinocytes of the small intestine.

Material and methods. The material for our research was the small intestine of 15 white outbred rats. Animals were sacrificed under etaminal sodium anesthesia. The small intestine of rats was rolled up and fixed in Bouin's fluid. To study the small intestine, the material was taken from various parts of the duodenum, jejunum and ileum. Cryostat sections from unfixed material were treated with a solution of glyoxylic acid to detect endocrinocytes containing fluorogenic amines. The ratio of catecholamines and serotonin was studied using the FMEL-1A attachment on a LUMAM-I2 luminescent microscope. Conducting and pouring the material into paraffin is performed according to the generally accepted method. Serial paraffin sections from the material were stained with hematoxylin-eosin according to Van Gieson and impregnated with silver nitrate according to the Grimelius method. The density of apudocytes was determined by the point method.